Concern 4. (c) creation of liquor by using micro organisms. (d) to make phony limbs, diagnostic devices instance ECG and EEG, etc. Answer: (b) hybridization away from DNA of one organism to that particular of one’s other people.

Matter 5. Check out the pursuing the statements: we. Recombinant DNA technology is often called hereditary technology is actually a great blast of biotechnology and this works together the newest manipulation out-of genetic information by the boy invitro ii. pBR322 is the first phony cloning vector designed in 1977 from the Boliver and you will Rodriguez regarding Elizabeth.coli plasmid. iii. Limit enzymes fall into a course off nutrients named nucleases. Choose the correct choice regarding the above comments (a) i and you may ii (b) we and you will iii (c) ii and you can iii (d) i,ii, and you may iii Answer: (d) i,ii and iii

Question 6. The process of recombinant DNA tech provides the after the measures i. Amplification of your own gene. ii. Installation out-of recombinant DNA on the server cells. iii. Cutting regarding DNA at the a particular escort girl Lakewood place having fun with a constraint enzyme. iv. Isolation of hereditary situation (DNA). Choose the correct sequence out-of steps to own recombinant DNA technology. (a) ii, iii, iv, and i (b) iv, ii, iii, and i also (c) i, ii, iii and you may iv (d) iv, iii, i, and you can ii Respond to: (d) iv, iii, we, and you will ii

Concern eight. What type of your after the palindromic foot sequences inside the DNA is easily be slashed around the guts by the some brand of restriction nutrients? (a) 5? CGTTCG step 3? ATCGTA5? (b) 5? GATATG 3? CTACTA5? (c) 5? GAATTC step 3? CTTAAG 5? (d) 5? CACGTA step 3? CTCAGT 5? Answer: (c) 5? GAATTC 3? CTTAAG 5?

Matter 12

Concern 8. pBR 322, BR signifies (a) Plasmid Bacterial Recombination (b) Plasmid Microbial Replications (c) Plasmid Boliver and you will Rodriguez (d) Plasmid Baltimore and you may Rodriguez Answer: (c) Plasmid Boliver and Rodriguez

Matter nine. And this of the pursuing the you’re used just like the an excellent Biosensors? (a) Electrophoresis (b) Bioreactors (c) Vectors (d) Electroporation Answer: (b) Bioreactors

(A) a-b-c d (B) c d b good (C) a c b d (D) c d a-b Answer: (D) c d a b

Where processes Ethidium Bromide is employed? (a) South Blotting processes (b) West Blotting process (c) Polymerase Chain Impulse (d) Agarose Serum Electrophoresis Respond to: (d) Agarose Serum Electrophoresis

Concern 11

Assertion: Agrobacterium tumifaciens was well-known inside the hereditary systems due to this fact germs try in the sources nodules of the many cereals and heart circulation vegetation. Reason: A great gene included on microbial chromosomal genome gets automatically transported on cross in which germs is actually associated. (a) One another denial and reasoning try correct. However, cause ’s the correct reasons of denial. (b) Both assertion and you may reason is genuine. However, reasoning is not a correct reason of denial. (c) Denial is true, but reason was incorrect. (d) Denial was false, but cause holds true. (e) Each other assertion and need are untrue. Answer: (a) Both denial and cause try true. But reasoning is correct reason off assertion.

Question thirteen. Which one of one’s following is not proper statement? (a) Ti plasmid causes the fresh bunchy best situation (b) Multiple cloning web site is called Polylinker (c) Non-widespread particular transfection regarding Nucleic acidic inside cell (d) Polylactic acidic is a type of biodegradable and you will bioactive thermoplastic. Answer: (a) Ti plasmid reasons brand new bunchy greatest condition

Question fourteen. A diagnosis away from chromosomal DNA using the southern hybridisation approach does maybe not have fun with (a) Electrophoresis (b) Blotting (c) Autoradiography (d) Polymerase Strings Impulse Respond to: (a) Electrophoresis

Question 15. An antibiotic gene inside the an excellent vector always facilitates the choice out-of (a) Skilled tissues (b) Turned tissue (c) Recombinant tissues (d) Nothing of your more than Respond to: (a) Competent cells

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